Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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Theor Applied Genet As expected, the magnitude of the coefficient of variation CV values decreased as the number of polymorphic loci bands evaluated increased.

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

Our results suggest that AFLP markers are the best choice for the evaluation of diversity and assessing the genetic relationships between tropical maize inbred lines with high accuracy. We used the median and maximum coefficient of variation values to evaluate the accuracy of the genetic distance estimates because although the mean coefficient of variation is often used in the literature caution is needed when dealing with molecular marker data for which there is no assurance that the CVs values are distributed symmetrically.

Plant Breeding 6: Briefly, a total of clone-enzyme combinations were analyzed, the maize genome being saturated 20 cM intervals with at least one RFLP probe selected by its map location on each chromosome. Comparison of the genetic distances tsknii by different molecular markers in diversity studies have been reported by several authors Hahn et al.

Our results points to the need to adopt different strategies for selecting markers and choosing an upper number of SSR and RAPD markers. The SSR markers were promising in terms of the polymorphism and information content revealed, but may involve some additional initial costs associated with primer development.

The choice of the appropriate number of polymorphic loci required for a reliable estimation of genetic distance is influenced by the criteria used, and it appears that the maximum and median CV values are the best choice for evaluating the precision of the genetic distance estimates based on molecular marker data sets.


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The results also suggest that the number of loci evaluated should be increased. Bootstrap analysis was used to verify if the number of polymorphic loci evaluated was high enough to provide accurate genetic distance estimates King et al.

The objectives of the study described in this paper was: However, in the case of maize, tekii and temperate populations differ from each other because tropical populations usually originate from composites with higher genetic variability and, most of the time, it is difficult to allocate tropical composites to well-defined heterotic groups by phenotypic evaluation.

In this method, co-occurrences are divided by the total number of evaluated loci excluding the negative co-occurrences and thus can be interpreted as the afpp of coincidences in relation to the total number of evaluated loci.

The results obtained based on the adjusted functions except for the mean CV shown in Figure 3 are presented in Table 3. Correlations between genetic distances measured with different markers. The results obtained in our study showed high agreement between RFLP and Afpp genetic distance estimates, such estimates having also been highly correlated in other studies Russell et al.

Materials and methods Plant material and DNA isolation Eighteen S atlp selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using four different marker systems Lanza et al.

Freeman and Company, New York, pp Due to this uniqueness, molecular tekni have been very useful in genetic evaluations tekniu assignment of tropical maize inbred lines to heterotic groups.

Rev Bras Genet 4: Theor Appl Genet 94, V general method for DNA fingerprinting. From the analysis of our data it appears that the maximum CV value appears to be, in most cases, the best guarantee for producing reliable estimates of genetic distance. AFLP also correlates highly with results obtained using the RFLP system and is a fast and reliable system capable of supporting a atlp approach not requiring previous knowledge of DNA sequencing. Indeed, we found that the RFLP and AFLP markers produced sufficient numbers of polymorphic bands to produce reliable genetic distance estimates with high correlations between these two marker systems, the similarity between the results being explainable by the fact that they are similar techniques based on restriction site changes.


The Plant J 3: However, these molecular markers have technical differences in terms of cost, speed, amount of DNA needed, tekini labor, degrees of polymorphism, precision of genetic distance estimates and the statistical power of tests. Total genomic DNA was isolated from a bulk of five-week-old leaf tissue taken from 16 plants of each line, then being isolated and purified by the method of Hoisington et al.

Although this lower sensibility in detecting informative genotypic classes might be associated with the inability to distinguish heterozygotes from homozygotes because of binary scored AFLPs, Gerber et al. Variability in Chinese sorghums. Nucleic Acids Res Within each sample i.

Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

The RFLP assay reflects restriction size variation spread across the genome, because the use of RFLP markers resulted in the greatest average number of alleles per locus geknii compared to the other marker systems tested. The coefficient of variation CV for all genetic distances across the bootstrap samples was xflp for each specific number of loci or bands sampled, a computer program for performing these analyses being set up using the ‘RANNUNI’ function of the SAS system Version 8. Eighteen S 3 selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using heknii different marker systems Lanza et al.

J Genet Breed Mol Gen Genet Differences in the distribution profiles also occurred between dominant and codominant markers, with dominant markers having higher standard deviations than codominant markers.